Affinity Chromatography Products

Affinity chromatography selection guide

To find the media or prepacked columns that suit your needs, choose the option below that best describes your application.

Affinity chromatography

I will purify an antibody or antibody fragment

I will purify a tagged recombinant protein

I will purify a protein that is not an antibody/antibody fragment or a tagged protein

Product	Product Name	Pack Size
17056901	EAH Sepharose 4B, 50 mL	50 mL	Get Quote

Product	Product Name	Pack Size
17048001	Epoxy-Activated Sepharose 6B, 15 g	15 g	Get Quote

Product	Product Name	Pack Size
17064001	Activated Thiol Sepharose 4B	15 g	Get Quote
17048001	Epoxy-Activated Sepharose 6B, 15 g	15 g	Get Quote
17042001	Thiopropyl Sepharose 6B, 15 g	15 g

Product	Product Name	Pack Size
17547801	Capto L, 25 mL	25 mL	Get Quote
17547806	Capto L, 5 mL	5 mL	Get Quote
17547802	Capto L, 200 mL	200 mL	Get Quote

Product	Product Name	Pack Size
17547851	HiTrap Protein L, 5 × 1 ml	5 × 1 ml	Get Quote
17547815	HiTrap Protein L, 1 × 5 ml	1 × 5 ml	Get Quote
29048665	HiTrap Protein L, 1 × 1 ml	1 × 1 ml	Get Quote
17547855	HiTrap Protein L, 5 × 5 ml	5 × 5 ml	Get Quote

Product	Product Name	Pack Size
17547801	Capto L, 25 mL	25 mL	Get Quote
17547806	Capto L, 5 mL	5 mL	Get Quote
17547802	Capto L, 200 mL	200 mL	Get Quote

Product	Product Name	Pack Size
17547851	HiTrap Protein L, 5 × 1 ml	5 × 1 ml	Get Quote
17547815	HiTrap Protein L, 1 × 5 ml	1 × 5 ml	Get Quote
29048665	HiTrap Protein L, 1 × 1 ml	1 × 1 ml	Get Quote
17547855	HiTrap Protein L, 5 × 5 ml	5 × 5 ml	Get Quote
17547814	HiScreen Capto L	1 × 4.7 mL	Get Quote

Product	Product Name	Pack Size
17513201	Glutathione Sepharose 4 Fast Flow, 25 mL	25 mL	Get Quote
17513202	Glutathione Sepharose 4 Fast Flow, 100 mL	100 mL	Get Quote
17513203	Glutathione Sepharose 4 Fast Flow, 500 mL	500 mL	Get Quote
17075601	Glutathione Sepharose 4B, 10 mL	10 mL	Get Quote
17075604	Glutathione Sepharose 4B, 300 mL	300 mL	Get Quote
17075605	Glutathione Sepharose 4B, 100 mL	100 mL	Get Quote
17527901	Glutathione Sepharose High Performance, 25 mL	25 mL	Get Quote
17527902	Glutathione Sepharose High Performance, 100 mL	100 mL	Get Quote
27457001	GST Bulk Kit	1 Kit	Get Quote

Product	Product Name	Pack Size
28405500	GST MultiTrap 4B	4 x 96-well filter plates	Get Quote
28952359	GST SpinTrap	50 x 50 µl	Get Quote
28952360	GST GraviTrap	10 x 2 ml	Get Quote
17513002	GSTrap FF	2 x 1 ml	Get Quote
17513005	GSTrap FF	100 x 1 ml
17513001	GSTrap FF	5 x 1 ml	Get Quote
28401746	GSTrap 4B, 100 x 1 ml	100 x 1 ml
28401745	GSTrap 4B, 5 x 1 ml	5 x 1 ml	Get Quote
29048609	GSTrap 4B, 1 x 1 ml	1 x 1 ml	Get Quote
17528101	GSTrap HP	5 x 1 ml	Get Quote
17528105	GSTrap HP	100 x 1 ml
17513101	GSTrap FF	1 x 5 ml	Get Quote
17513102	GSTrap FF	5 x 5 ml	Get Quote
17513105	GSTrap FF	100 x 5 ml
28401748	GSTrap 4B, 5 x 5 ml	5 x 5 ml	Get Quote
28401747	GSTrap 4B, 1 x 5 ml	1 x 5 ml	Get Quote
28401749	GSTrap 4B, 100 x 5 ml	100 x 5 ml
17528201	GSTrap HP	1 x 5 ml	Get Quote
17528202	GSTrap HP	5 x 5 ml	Get Quote
17528205	GSTrap HP	100 x 5 ml

Product	Product Name	Pack Size
17513201	Glutathione Sepharose 4 Fast Flow, 25 mL	25 mL	Get Quote
17513202	Glutathione Sepharose 4 Fast Flow, 100 mL	100 mL	Get Quote
17513203	Glutathione Sepharose 4 Fast Flow, 500 mL	500 mL	Get Quote
17075601	Glutathione Sepharose 4B, 10 mL	10 mL	Get Quote
17075604	Glutathione Sepharose 4B, 300 mL	300 mL	Get Quote
17075605	Glutathione Sepharose 4B, 100 mL	100 mL	Get Quote
17527901	Glutathione Sepharose High Performance, 25 mL	25 mL	Get Quote
17527902	Glutathione Sepharose High Performance, 100 mL	100 mL	Get Quote

Product	Product Name	Pack Size
17513002	GSTrap FF	2 x 1 ml	Get Quote
17513005	GSTrap FF	100 x 1 ml
17513001	GSTrap FF	5 x 1 ml	Get Quote
28401746	GSTrap 4B, 100 x 1 ml	100 x 1 ml
28401745	GSTrap 4B, 5 x 1 ml	5 x 1 ml	Get Quote
29048609	GSTrap 4B, 1 x 1 ml	1 x 1 ml	Get Quote
17528101	GSTrap HP	5 x 1 ml	Get Quote
17528105	GSTrap HP	100 x 1 ml
17513101	GSTrap FF	1 x 5 ml	Get Quote
17513102	GSTrap FF	5 x 5 ml	Get Quote
17513105	GSTrap FF	100 x 5 ml
28401748	GSTrap 4B, 5 x 5 ml	5 x 5 ml	Get Quote
28401747	GSTrap 4B, 1 x 5 ml	1 x 5 ml	Get Quote
28401749	GSTrap 4B, 100 x 5 ml	100 x 5 ml
17528201	GSTrap HP	1 x 5 ml	Get Quote
17528202	GSTrap HP	5 x 5 ml	Get Quote
17528205	GSTrap HP	100 x 5 ml
28936550	GSTPrep FF 16/10	20 ml	Get Quote

Product	Product Name	Pack Size
28935600	StrepTactin Sepharose High Performance, 50 mL	50 mL	Get Quote

Product	Product Name	Pack Size
28907546	StrepTrap HP, 5 x 1 ml	5 x 1 ml	Get Quote
29048653	StrepTrap HP, 1 x 1 ml	1 x 1 ml	Get Quote
28907548	StrepTrap HP, 5 x 5 ml	5 x 5 ml	Get Quote
28907547	StrepTrap HP, 1 x 5 ml	1 x 5 ml	Get Quote

Product	Product Name	Pack Size
28935600	StrepTactin Sepharose High Performance, 50 mL	50 mL	Get Quote

Product	Product Name	Pack Size
28907546	StrepTrap HP, 5 x 1 ml	5 x 1 ml	Get Quote
29048653	StrepTrap HP, 1 x 1 ml	1 x 1 ml	Get Quote
28907548	StrepTrap HP, 5 x 5 ml	5 x 5 ml	Get Quote
28907547	StrepTrap HP, 1 x 5 ml	1 x 5 ml	Get Quote

Product	Product Name	Pack Size
28935597	Dextrin Sepharose High Performance, 25 mL	25 mL	Get Quote
28935598	Dextrin Sepharose High Performance, 100 mL	100 mL	Get Quote

Product	Product Name	Pack Size
28918778	MBPTrap HP, 5 x 1 ml	5 x 1 ml	Get Quote
29048641	MBPTrap HP, 1 x 1 ml	1 x 1 ml	Get Quote
28918779	MBPTrap HP, 1 x 5 ml	1 x 5 ml	Get Quote
28918780	MBPTrap HP, 5 x 5 ml	5 x 5 ml	Get Quote

Product	Product Name	Pack Size
28935597	Dextrin Sepharose High Performance, 25 mL	25 mL	Get Quote
28935598	Dextrin Sepharose High Performance, 100 mL	100 mL	Get Quote

Product	Product Name	Pack Size
28918778	MBPTrap HP, 5 x 1 ml	5 x 1 ml	Get Quote
29048641	MBPTrap HP, 1 x 1 ml	1 x 1 ml	Get Quote
28918779	MBPTrap HP, 1 x 5 ml	1 x 5 ml	Get Quote
28918780	MBPTrap HP, 5 x 5 ml	5 x 5 ml	Get Quote

Product	Product Name	Pack Size
28985738	Streptavidin Mag Sepharose	2 x 1 ml 10% slurry	Get Quote
28985799	Streptavidin Mag Sepharose	5 x 1 ml 10% slurry	Get Quote
28903131	Streptavidin HP MultiTrap	4	Get Quote
28903130	Streptavidin HP SpinTrap	16 Columns	Get Quote
17511201	HiTrap Streptavidin HP	5 x 1 ml	Get Quote

Product	Product Name	Pack Size
17127901	rProtein A Sepharose Fast Flow	5 mL	Get Quote
17127902	rProtein A Sepharose Fast Flow	25 mL	Get Quote
17127903	rProtein A Sepharose Fast Flow	200 mL	Get Quote

Product	Product Name	Pack Size
28967056	Protein A Mag Sepharose Xtra	2 x 1 ml	Get Quote
28967062	Protein A Mag Sepharose Xtra	5 x 1 ml	Get Quote
28903133	Protein A HP MultiTrap	4	Get Quote
28903132	Protein A HP SpinTrap	16 Columns	Get Quote
28985254	rProtein A GraviTrap		Get Quote
17040201	HiTrap Protein A HP, 5 x 1 ml	5 x 1 ml	Get Quote
17040203	HiTrap Protein A HP, 2 x 1 ml	2 x 1 ml	Get Quote
29048576	HiTrap Protein A HP, 1 x 1 ml	1 x 1 ml	Get Quote
28946489	HiTrap rProtein A FF 100 x 1 ml	100 x 1 ml
17507901	HiTrap rProtein A FF, 5 x 1 ml	5 x 1 ml	Get Quote
17507902	HiTrap rProtein A FF, 2 x 1 ml	2 x 1 ml	Get Quote
11003493	HiTrap MabSelect SuRe, 5 x 1 ml	5 x 1 ml	Get Quote
29049104	HiTrap MabSelect SuRe, 1 x 1 ml	1 x 1 ml	Get Quote
17549111	HiTrap MabSelect SuRe pcc, 5 x 1 ml	5 x 1 ml	Get Quote
17040301	HiTrap Protein A HP, 1 x 5 ml	1 x 5 ml	Get Quote
17040303	HiTrap Protein A HP, 5 x 5 ml	5 x 5 ml	Get Quote
17040503	HiTrap Protein G HP, 5 x 5 ml	5 x 5 ml	Get Quote
17508002	HiTrap rProtein A FF, 5 x 5 ml	5 x 5 ml	Get Quote
17508001	HiTrap rProtein A FF, 1 x 5 ml	1 x 5 ml	Get Quote
11003494	HiTrap MabSelect SuRe, 1 x 5 ml	1 x 5 ml	Get Quote
11003495	HiTrap MabSelect SuRe, 5 x 5 ml	5 x 5 ml	Get Quote
17549112	HiTrap MabSelect SuRe pcc, 1 x 5 ml	1 x 5 ml	Get Quote

Product	Product Name	Pack Size
17127901	rProtein A Sepharose Fast Flow	5 mL	Get Quote
17127902	rProtein A Sepharose Fast Flow	25 mL	Get Quote
17127903	rProtein A Sepharose Fast Flow	200 mL	Get Quote
17543801	MabSelect SuRe, 25 mL	25 mL	Get Quote
17543802	MabSelect SuRe, 200 mL	200 mL	Get Quote
17547401	MabSelect SuRe LX, 25 mL	25 mL	Get Quote
17547402	MabSelect SuRe LX, 200 mL	200 mL	Get Quote
17549101	MabSelect SuRe pcc, 25 mL	25 mL	Get Quote
17549102	MabSelect SuRe pcc, 200 mL	200 mL	Get Quote

Product	Product Name	Pack Size
17040201	HiTrap Protein A HP, 5 x 1 ml	5 x 1 ml	Get Quote
17040203	HiTrap Protein A HP, 2 x 1 ml	2 x 1 ml	Get Quote
29048576	HiTrap Protein A HP, 1 x 1 ml	1 x 1 ml	Get Quote
28946489	HiTrap rProtein A FF 100 x 1 ml	100 x 1 ml
17507901	HiTrap rProtein A FF, 5 x 1 ml	5 x 1 ml	Get Quote
17507902	HiTrap rProtein A FF, 2 x 1 ml	2 x 1 ml	Get Quote
11003493	HiTrap MabSelect SuRe, 5 x 1 ml	5 x 1 ml	Get Quote
29049104	HiTrap MabSelect SuRe, 1 x 1 ml	1 x 1 ml	Get Quote
17549111	HiTrap MabSelect SuRe pcc, 5 x 1 ml	5 x 1 ml	Get Quote
17040301	HiTrap Protein A HP, 1 x 5 ml	1 x 5 ml	Get Quote
17040303	HiTrap Protein A HP, 5 x 5 ml	5 x 5 ml	Get Quote
17040503	HiTrap Protein G HP, 5 x 5 ml	5 x 5 ml	Get Quote
17508002	HiTrap rProtein A FF, 5 x 5 ml	5 x 5 ml	Get Quote
17508001	HiTrap rProtein A FF, 1 x 5 ml	1 x 5 ml	Get Quote
11003494	HiTrap MabSelect SuRe, 1 x 5 ml	1 x 5 ml	Get Quote
11003495	HiTrap MabSelect SuRe, 5 x 5 ml	5 x 5 ml	Get Quote
17549112	HiTrap MabSelect SuRe pcc, 1 x 5 ml	1 x 5 ml	Get Quote
28926977	HiScreen MabSelect SuRe	1 × 4.7 mL	Get Quote
17547415	HiScreen MabSelect SuRe LX	1 × 4.7 mL	Get Quote

Product	Product Name	Pack Size
17061801	Protein G Sepharose 4 Fast Flow	5 mL	Get Quote
17061805	Protein G Sepharose 4 Fast Flow	200 mL	Get Quote
17061802	Protein G Sepharose 4 Fast Flow	25 mL	Get Quote
17088601	GammaBind Plus Sepharose, 5 mL	5 mL	Get Quote
17088602	GammaBind Plus Sepharose, 25 mL	25 mL	Get Quote
17088604	GammaBind Plus Sepharose, 500 mL	500 mL	Get Quote

Product	Product Name	Pack Size
28903135	Protein G HP MultiTrap	4	Get Quote
28408347	Ab SpinTrap	50 x 100 µl	Get Quote
28903134	Protein G HP SpinTrap	16 Columns	Get Quote
28985255	Protein G GraviTrap		Get Quote
28985256	rProtein A Protein G GraviTrap		Get Quote
17040403	HiTrap Protein G HP, 2 x 1 ml	2 x 1 ml	Get Quote
17040401	HiTrap Protein G HP, 5 x 1 ml	5 x 1 ml	Get Quote
29048581	HiTrap Protein G HP, 1 x 1 ml	1 x 1 ml	Get Quote
17040501	HiTrap Protein G HP, 1 x 5 ml	1 x 5 ml	Get Quote
17040503	HiTrap Protein G HP, 5 x 5 ml	5 x 5 ml	Get Quote

Product	Product Name	Pack Size
17061801	Protein G Sepharose 4 Fast Flow	5 mL	Get Quote
17061805	Protein G Sepharose 4 Fast Flow	200 mL	Get Quote
17061802	Protein G Sepharose 4 Fast Flow	25 mL	Get Quote
17088601	GammaBind Plus Sepharose, 5 mL	5 mL	Get Quote
17088602	GammaBind Plus Sepharose, 25 mL	25 mL	Get Quote
17088604	GammaBind Plus Sepharose, 500 mL	500 mL	Get Quote

Product	Product Name	Pack Size
17040403	HiTrap Protein G HP, 2 x 1 ml	2 x 1 ml	Get Quote
17040401	HiTrap Protein G HP, 5 x 1 ml	5 x 1 ml	Get Quote
29048581	HiTrap Protein G HP, 1 x 1 ml	1 x 1 ml	Get Quote
17040501	HiTrap Protein G HP, 1 x 5 ml	1 x 5 ml	Get Quote
17040503	HiTrap Protein G HP, 5 x 5 ml	5 x 5 ml	Get Quote
17511001	HiTrap IgM Purification HP	5 x 1 ml	Get Quote
17511101	HiTrap IgY Purification HP	5 ml	Get Quote

Product	Product Name	Pack Size
17531801	Ni Sepharose 6 Fast Flow, 25 mL	25 mL	Get Quote
17531806	Ni Sepharose 6 Fast Flow, 5 mL	5 mL	Get Quote
17531803	Ni Sepharose 6 Fast Flow, 500 mL	500 mL	Get Quote
17531802	Ni Sepharose 6 Fast Flow, 100 mL	100 mL	Get Quote
17526801	Ni Sepharose High Performance, 25 mL	25 mL	Get Quote
17526802	Ni Sepharose High Performance, 100 mL	100 mL	Get Quote
28957499	TALON Superflow, 10 mL	10 mL	Get Quote
28957502	TALON Superflow, 50 mL	50 mL	Get Quote
17371201	Ni Sepharose excel, 25 mL	25 mL	Get Quote
17371202	Ni Sepharose excel, 100 mL	100 mL	Get Quote
17371203	Ni Sepharose excel, 500 mL	500 mL	Get Quote

Product	Product Name	Pack Size
11000458	HisTrap FF Crude, 5 x 1 ml	5 x 1 ml	Get Quote
11000459	HisTrap FF Crude, 100 x 1 ml	100 x 1 ml
11003399	His GraviTrap	10 x 1 ml	Get Quote
17371205	HisTrap excel, 5 x 1 ml	5 x 1 ml	Get Quote
17371206	HisTrap excel, 5 x 5 ml	5 x 5 ml	Get Quote
17371220	His Mag Sepharose excel	2 x 1 ml	Get Quote
17371221	His Mag Sepharose excel	5 x 1 ml	Get Quote
17371222	His Mag Sepharose excel	10 x 1 ml	Get Quote
17524701	HisTrap HP, 5 x 1 ml	5 x 1 ml	Get Quote
17524705	HisTrap HP, 100 x 1 ml	100 x 1 ml
17524801	HisTrap HP, 1 x 5 ml	1 x 5 ml	Get Quote
17524802	HisTrap HP, 5 x 5 ml	5 x 5 ml	Get Quote
17524805	HisTrap HP, 100 x 5 ml	100 x 5 ml
17525501	HisTrap FF	5 x 5 ml	Get Quote
17525502	HisTrap FF	100 x 5 ml
17528601	HisTrap FF Crude, 5 x 5 ml	5 x 5 ml	Get Quote
17528602	HisTrap FF Crude, 100 x 5 ml	100 x 5 ml
17531901	HisTrap FF	5 x 1 ml	Get Quote
17531902	HisTrap FF	100 x 1 ml
28400989	His MultiTrap HP	4 x 96-well filter plates	Get Quote
28400990	His MultiTrap FF	4 x 96-well filter plates	Get Quote
28401351	His GraviTrap Kit	10 Columns	Get Quote
28401353	His SpinTrap	50 x 100 µl	Get Quote
28932171	His SpinTrap Kit	1 Piece	Get Quote
28953766	HiTrap TALON crude, 5 x 1 ml	5 x 1 ml	Get Quote
28953767	HiTrap TALON crude, 5 x 5 ml	5 x 5 ml	Get Quote
28953805	HiTrap TALON crude, 100 x 1 ml	100 x 1 ml
28953809	HiTrap TALON crude, 100 x 5 ml	100 x 5 ml
28967388	His Mag Sepharose Ni	2 x 1 ml	Get Quote
28967390	His Mag Sepharose Ni	5 x 1 ml	Get Quote
28979917	His Mag Sepharose Ni	10 x 1 ml	Get Quote
29000593	His SpinTrap TALON	50 x 100 μl	Get Quote
29000594	His GraviTrap TALON	10	Get Quote
29000596	His MultiTrap TALON	4 x 96-well filter plates	Get Quote
29048565	HiTrap TALON crude, 1 x 1 ml	1 x 1 ml	Get Quote
29048586	HisTrap excel, 1 x 1 ml	1 x 1 ml	Get Quote
29048631	HisTrap FF Crude, 1 x 1 ml	1 x 1 ml	Get Quote
29051021	HisTrap HP, 1 x 1 ml	1 x 1 ml	Get Quote
29104065	His Mag Sepharose Ni	100 ml	Get Quote

Product	Product Name	Pack Size
17531801	Ni Sepharose 6 Fast Flow, 25 mL	25 mL	Get Quote
17531806	Ni Sepharose 6 Fast Flow, 5 mL	5 mL	Get Quote
17531803	Ni Sepharose 6 Fast Flow, 500 mL	500 mL	Get Quote
17531802	Ni Sepharose 6 Fast Flow, 100 mL	100 mL	Get Quote
17526801	Ni Sepharose High Performance, 25 mL	25 mL	Get Quote
17526802	Ni Sepharose High Performance, 100 mL	100 mL	Get Quote
28957499	TALON Superflow, 10 mL	10 mL	Get Quote
28957502	TALON Superflow, 50 mL	50 mL	Get Quote
17371201	Ni Sepharose excel, 25 mL	25 mL	Get Quote
17371202	Ni Sepharose excel, 100 mL	100 mL	Get Quote
17371203	Ni Sepharose excel, 500 mL	500 mL	Get Quote

Product	Product Name	Pack Size
29048631	HisTrap FF Crude, 1 x 1 ml	1 x 1 ml	Get Quote
11000458	HisTrap FF Crude, 5 x 1 ml	5 x 1 ml	Get Quote
11000459	HisTrap FF Crude, 100 x 1 ml	100 x 1 ml
29048565	HiTrap TALON crude, 1 x 1 ml	1 x 1 ml	Get Quote
28953766	HiTrap TALON crude, 5 x 1 ml	5 x 1 ml	Get Quote
17528601	HisTrap FF Crude, 5 x 5 ml	5 x 5 ml	Get Quote
17528602	HisTrap FF Crude, 100 x 5 ml	100 x 5 ml
28953809	HiTrap TALON crude, 100 x 5 ml	100 x 5 ml
17531901	HisTrap FF	5 x 1 ml	Get Quote
17531902	HisTrap FF	100 x 1 ml
29051021	HisTrap HP, 1 x 1 ml	1 x 1 ml	Get Quote
17524701	HisTrap HP, 5 x 1 ml	5 x 1 ml	Get Quote
17524705	HisTrap HP, 100 x 1 ml	100 x 1 ml
17371205	HisTrap excel, 5 x 1 ml	5 x 1 ml	Get Quote
29048586	HisTrap excel, 1 x 1 ml	1 x 1 ml	Get Quote
28953805	HiTrap TALON crude, 100 x 1 ml	100 x 1 ml
17525501	HisTrap FF	5 x 5 ml	Get Quote
17525502	HisTrap FF	100 x 5 ml
17524801	HisTrap HP, 1 x 5 ml	1 x 5 ml	Get Quote
17524802	HisTrap HP, 5 x 5 ml	5 x 5 ml	Get Quote
17524805	HisTrap HP, 100 x 5 ml	100 x 5 ml
17371206	HisTrap excel, 5 x 5 ml	5 x 5 ml	Get Quote
28953767	HiTrap TALON crude, 5 x 5 ml	5 x 5 ml	Get Quote
28936551	HisPrep FF 16/10	20 ml	Get Quote
28978244	HiScreen Ni FF	4.7 ml	Get Quote

Product	Product Name	Pack Size
17092107	IMAC Sepharose 6 Fast Flow, 25 mL	25 mL	Get Quote
17092108	IMAC Sepharose 6 Fast Flow, 100 mL	100 mL	Get Quote
17092007	IMAC Sepharose High Performance, 100 mL	100 mL	Get Quote

Product	Product Name	Pack Size
17092102	HiTrap IMAC FF	5 x 1 ml	Get Quote
17092003	HiTrap IMAC HP	5 x 1 ml	Get Quote
17092104	HiTrap IMAC FF	5 x 5 ml	Get Quote
17092005	HiTrap IMAC HP	5 x 5 ml	Get Quote

Product	Product Name	Pack Size
17092107	IMAC Sepharose 6 Fast Flow, 25 mL	25 mL	Get Quote
17092108	IMAC Sepharose 6 Fast Flow, 100 mL	100 mL	Get Quote
17092007	IMAC Sepharose High Performance, 100 mL	100 mL	Get Quote

Product	Product Name	Pack Size
17092102	HiTrap IMAC FF	5 x 1 ml	Get Quote
17092003	HiTrap IMAC HP	5 x 1 ml	Get Quote
17092104	HiTrap IMAC FF	5 x 5 ml	Get Quote
17092005	HiTrap IMAC HP	5 x 5 ml	Get Quote
28936552	HiPrep IMAC FF 16/10	20 ml	Get Quote
28978244	HiScreen Ni FF	4.7 ml	Get Quote

Product	Product Name	Pack Size
17511301	Streptavidin Sepharose High Performance, 5 mL	5 mL	Get Quote

Product	Product Name	Pack Size
17511201	HiTrap Streptavidin HP	5 x 1 ml	Get Quote

Product	Product Name	Pack Size
17044401	Lentil Lectin Sepharose 4B, 25 mL	25 mL	Get Quote
17044001	Con A Sepharose 4B, 100 mL	100 mL	Get Quote
17044003	Con A Sepharose 4B, 5 mL	5 mL	Get Quote

Product	Product Name	Pack Size
28952085	HiTrap Con A 4B	5 x 1 ml	Get Quote
17044003	Con A Sepharose 4B, 5 mL	5 mL	Get Quote
28952096	HiTrap Con A 4B	5 x 5 ml	Get Quote

Product	Product Name	Pack Size
17044401	Lentil Lectin Sepharose 4B, 25 mL	25 mL	Get Quote
17044001	Con A Sepharose 4B, 100 mL	100 mL	Get Quote
17044003	Con A Sepharose 4B, 5 mL	5 mL	Get Quote

Product	Product Name	Pack Size
28952085	HiTrap Con A 4B	5 x 1 ml	Get Quote
17044003	Con A Sepharose 4B, 5 mL	5 mL	Get Quote
28952096	HiTrap Con A 4B	5 x 5 ml	Get Quote

Product	Product Name	Pack Size
17094801	Blue Sepharose 6 Fast Flow, 50 mL	50 mL	Get Quote
17070001	2'5' ADP Sepharose 4B	5 g	Get Quote
17095601	Gelatin Sepharose 4B	25 mL	Get Quote
17099801	Heparin Sepharose 6 Fast Flow	50 mL	Get Quote
17512310	Benzamidine Sepharose 4 Fast Flow (High Sub), 25 mL	25 mL	Get Quote
17092107	IMAC Sepharose 6 Fast Flow, 25 mL	25 mL	Get Quote
17092108	IMAC Sepharose 6 Fast Flow, 100 mL	100 mL	Get Quote
17092007	IMAC Sepharose High Performance, 100 mL	100 mL	Get Quote
17096901	IgG Sepharose 6 Fast Flow, 10 mL	10 mL	Get Quote
17052901	Calmodulin Sepharose 4B, 10 mL	10 mL	Get Quote

Product	Product Name	Pack Size
17041201	HiTrap Blue HP	5 x 1 ml	Get Quote
17041301	HiTrap Blue HP	5 ml	Get Quote
17040601	HiTrap Heparin HP 5 x 1 ml	5 x 1 ml	Get Quote
17040701	HiTrap Heparin HP 1 x 5 ml	1 x 5 ml	Get Quote
17040703	HiTrap Heparin HP 5 x 5 ml	5 x 5 ml	Get Quote
17514301	HiTrap Benzamidine FF (High Sub), 5 x 1ml	5 x 1 ml	Get Quote
17514302	HiTrap Benzamidine FF (High Sub), 2 x 1ml	2 x 1 ml	Get Quote
17514401	HiTrap Benzamidine FF (High Sub), 1 x 5ml	1 x 5 ml	Get Quote
17092102	HiTrap IMAC FF	5 x 1 ml	Get Quote
17092104	HiTrap IMAC FF	5 x 5 ml	Get Quote
17092003	HiTrap IMAC HP	5 x 1 ml	Get Quote
17092005	HiTrap IMAC HP	5 x 5 ml	Get Quote
90100397	HiTrap IgG Sepharose 6 FF	5 x 1 ml	Get Quote
28908366	HiTrap IgG Sepharose FF	5 x 5 ml	Get Quote

Product	Product Name	Pack Size
17094801	Blue Sepharose 6 Fast Flow, 50 mL	50 mL	Get Quote
17544801	Capto Blue, 25 mL	25 mL	Get Quote
17070001	2'5' ADP Sepharose 4B	5 g	Get Quote
17095601	Gelatin Sepharose 4B	25 mL	Get Quote
17099801	Heparin Sepharose 6 Fast Flow	50 mL	Get Quote
17512310	Benzamidine Sepharose 4 Fast Flow (High Sub), 25 mL	25 mL	Get Quote
17092107	IMAC Sepharose 6 Fast Flow, 25 mL	25 mL	Get Quote
17092108	IMAC Sepharose 6 Fast Flow, 100 mL	100 mL	Get Quote
17092007	IMAC Sepharose High Performance, 100 mL	100 mL	Get Quote
17096901	IgG Sepharose 6 Fast Flow, 10 mL	10 mL	Get Quote
17052901	Calmodulin Sepharose 4B, 10 mL	10 mL	Get Quote

Product	Product Name	Pack Size
17041201	HiTrap Blue HP	5 x 1 ml	Get Quote
17041301	HiTrap Blue HP	5 ml	Get Quote
28992474	HiScreen Capto Blue		Get Quote
17040601	HiTrap Heparin HP 5 x 1 ml	5 x 1 ml	Get Quote
17040701	HiTrap Heparin HP 1 x 5 ml	1 x 5 ml	Get Quote
17040703	HiTrap Heparin HP 5 x 5 ml	5 x 5 ml	Get Quote
28936549	HiPrep Heparin FF 16/10	20 ml	Get Quote
17514301	HiTrap Benzamidine FF (High Sub), 5 x 1ml	5 x 1 ml	Get Quote
17514302	HiTrap Benzamidine FF (High Sub), 2 x 1ml	2 x 1 ml	Get Quote
17514401	HiTrap Benzamidine FF (High Sub), 1 x 5ml	1 x 5 ml	Get Quote
17092102	HiTrap IMAC FF	5 x 1 ml	Get Quote
17092104	HiTrap IMAC FF	5 x 5 ml	Get Quote
17092003	HiTrap IMAC HP	5 x 1 ml	Get Quote
17092005	HiTrap IMAC HP	5 x 5 ml	Get Quote
28936552	HiPrep IMAC FF 16/10	20 ml	Get Quote
90100397	HiTrap IgG Sepharose 6 FF	5 x 1 ml	Get Quote
28908366	HiTrap IgG Sepharose FF	5 x 5 ml	Get Quote

Product	Product Name	Pack Size
17090601	NHS-Activated Sepharose 4 Fast Flow, 25 mL	25 mL	Get Quote
17043001	CNBr-Activated Sepharose 4B, 15 g	15 g	Get Quote
17098101	CNBr-Activated Sepharose 4 Fast Flow, 10 g	10 g	Get Quote

Product	Product Name	Pack Size
28903128	NHS HP SpinTrap	-	Get Quote
28944009	NHS Mag Sepharose	500 μl	Get Quote
28951380	NHS Mag Sepharose	4 x 500 μl	Get Quote
17071601	HiTrap NHS-Activated HP, 5 x 1 ml	5 x 1 ml	Get Quote
17071630	HiTrap NHS-Activated HP, 180 x 1 ml	180 x 1 ml
17071701	HiTrap NHS-Activated HP, 1 x 5 ml	1 x 5 ml	Get Quote

Product	Product Name	Pack Size
17090601	NHS-Activated Sepharose 4 Fast Flow, 25 mL	25 mL	Get Quote
17043001	CNBr-Activated Sepharose 4B, 15 g	15 g	Get Quote
17098101	CNBr-Activated Sepharose 4 Fast Flow, 10 g	10 g	Get Quote

Product	Product Name	Pack Size
17071601	HiTrap NHS-Activated HP, 5 x 1 ml	5 x 1 ml	Get Quote
17071630	HiTrap NHS-Activated HP, 180 x 1 ml	180 x 1 ml
17071701	HiTrap NHS-Activated HP, 1 x 5 ml	1 x 5 ml	Get Quote

What types of products does GE offer for affinity chromatography?

We offer products for affinity purification of:

Tagged proteins: histidine-tagged (his-tagged), glutathione S-transferase (GST)-tagged, maltose binding protein (MBP)-tagged, Strep II-tag tagged.
Antibodies and antibody fragments.
Proteins that have affinity for specific groups of molecules. We provide options for affinity purification (enrichment) or removal of proteins containing biotin or carbohydrate groups. Media for affinity purification of other proteins are also available. Please see our selection tool for options.
Proteins with affinity for a ligand that can be coupled to GE pre-activated media. Options for coupling of a ligand via an amino, thiol, carboxyl, or hydroxyl group are available.

What types of affinity products does GE offer for histidine-tagged protein purification?

We offer a variety of products containing IMAC media precharged with nickel or cobalt ions. These products include prepacked columns for use with chromatography systems, prepacked formats for manual purification, and loose media (resins). Options containing uncharged IMAC media are also available. We recommend products containing the following media types based on your purification priorities:

High purity: TALON Superflow, which is precharged with cobalt ions
Avoiding nickel leakage: Ni Sepharose excel, which is precharged with nickel ions
High binding capacity: Ni Sepharose High Performance, which is precharged with nickel ions
Ability to scale up purification: Ni Sepharose 6 Fast Flow, which is precharged with nickel ions
Flexibility in metal ions used: Uncharged IMAC media

How do I choose between precharged and uncharged media for histidine-tagged protein purification?

The choice of metal ion affects binding specificity and strength. The requirements for this purification step will help with this choice. Nickel (Ni²⁺) is the most popular metal ion for histidine-tagged (his-tagged) protein purification. But nickel tends to bind nonspecifically to host proteins containing histidine clusters. Cobalt (Co²⁺) interacts more specifically with his-tags, often giving a more pure protein. GE offers many products containing media precharged with nickel or cobalt ions. Uncharged IMAC media are flexible options for preparing media charged with metal ions other than nickel or cobalt, such as zinc or copper.

Does my sample always have to be clarified before histidine-tagged protein purification using a column?

Before applying a crude sample to an IMAC column, the sample is typically clarified using centrifugation and filtration. An alternative is to apply an unclarified, crude sample to an appropriate column directly after thorough cell disruption. This option can save time and help to minimize protein degradation. GE provides a wide range of products that have been designed to purify histidine-tagged proteins from unclarified samples.

Should I be concerned about metal ion stripping during histidine-tagged protein purification?

Mammalian (e.g., CHO cells) and insect cell culture media contain substances that could reduce the yield of purified histidine-tagged (his-tagged) proteins. These components lower the binding to the IMAC medium by stripping the chelated metal ion. To improve protein yield, the sample can be pretreated to remove the interfering substances. Another option is to use a medium (e.g., Ni Sepharose excel) that binds to his-tagged proteins in the presence of metal stripping agents.

What types of products does GE offer for affinity purification of antibodies and antibody fragments?

Our product offering includes a range of agarose-based media with protein A, protein G, or protein L as ligand. We provide a variety of protein A- and G-based products for purifying monoclonal and polyclonal antibodies from cell culture supernatants and serum. Capto L is our medium for purifying antibody fragments that contain a kappa light chain. These fragments include single-chain fragment variable (scFv), Fab, and domain antibodies (Dab). Most of our products are available as prepacked columns for use with chromatography systems, prepacked formats for manual purification, and loose media (resins). We also offer prepacked columns for IgM and IgY purification, which are based on thiophilic adsorption.

Please use our online selection tool to find a product that meets your needs.

How do I choose between affinity media based on protein A or protein G?

Both protein A and protein G have high affinity and specificity for the Fc region of antibodies. However, they differ in their ability to bind antibodies of different species and subclasses (see table). We recommend protein A for purifying human antibodies. Protein G binds a broader range of IgG from eukaryotic species and binds more classes of IgG than protein A. Therefore, we recommend our protein G-based media to purify antibodies from other species, including rat. If you are not sure whether your antibody will bind to protein A or protein G, we suggest trying rProtein A/Protein G GraviTrap, which contains gravity flow columns packed with a combination of both media.

The table below shows the relative binding strengths of antibodies from various species to protein G and protein A.

Relative binding strengths of antibodies from various species to protein G and protein A as measured in a competitive ELISA test. The amount of IgG required to give a 50% inhibition of binding of rabbit IgG conjugated with alkaline phosphatase was determined.

Species	Subclass	Protein G binding	Protein A binding
* Purified using HiTrap IgM Purification HP columns ^† Purified using HiTrap IgY Purification HP columns ++++ = strong binding ++ = medium binding — = weak or no binding
Human	IgA	—	variable
	IgD	—	—
	IgE	—	—
	IgG₁	++++	++++
	IgG₂	++++	++++
	IgG₃	++++	—
	IgM*	—	variable
	IgG₄	++++	++++
Avian egg yolk	IgY^†	—	—
Cow		++++	++
Dog		+	++
Goat		++	—
Guinea pig	IgG₁	++	++++
Hamster		++	+
Horse		++++	++
Koala		+	—
Llama		+	—
Monkey (rhesus)		++++	++++
Mouse	IgG₁	++++	+
	IgG_2a	++++	++++
	IgG₂	+++	+++
	IgG₃	+++	++
	IgM*	—	variable
Pig		+++	+++
Rabbit		+++	++++
Rat	IgG₁	+	—
	IgG_2a	++++	—
	IgG_2b	++	—
	IgG₃	++	+
Sheep		++	+/—