Antibody Purification Products - GE Healthcare Life Sciences

Antibody purification selection guide

To find the media or prepacked columns that suit your needs, choose the option below that best describes your application.

Antibody purification

I will purify an antibody

I will purify an antibody fragment

Product	Product Name	Pack Size
17547801	Capto L, 25 mL	25 mL	Get Quote
17547806	Capto L, 5 mL	5 mL	Get Quote
17547802	Capto L, 200 mL	200 mL	Get Quote

Product	Product Name	Pack Size
17547851	HiTrap Protein L, 5 × 1 ml	5 × 1 ml	Get Quote
17547815	HiTrap Protein L, 1 × 5 ml	1 × 5 ml	Get Quote
29048665	HiTrap Protein L, 1 × 1 ml	1 × 1 ml	Get Quote
17547855	HiTrap Protein L, 5 × 5 ml	5 × 5 ml	Get Quote

Product	Product Name	Pack Size
17547801	Capto L, 25 mL	25 mL	Get Quote
17547806	Capto L, 5 mL	5 mL	Get Quote
17547802	Capto L, 200 mL	200 mL	Get Quote

Product	Product Name	Pack Size
17547851	HiTrap Protein L, 5 × 1 ml	5 × 1 ml	Get Quote
17547815	HiTrap Protein L, 1 × 5 ml	1 × 5 ml	Get Quote
29048665	HiTrap Protein L, 1 × 1 ml	1 × 1 ml	Get Quote
17547855	HiTrap Protein L, 5 × 5 ml	5 × 5 ml	Get Quote
17547814	HiScreen Capto L	1 × 4.7 mL	Get Quote

Product	Product Name	Pack Size
17127901	rProtein A Sepharose Fast Flow	5 mL	Get Quote
17127902	rProtein A Sepharose Fast Flow	25 mL	Get Quote
17127903	rProtein A Sepharose Fast Flow	200 mL	Get Quote

Product	Product Name	Pack Size
17549852	HiTrap MabSelect PrismA 5 x 1 mL	5 × 1 mL	Get Quote
17549853	HiTrap MabSelect PrismA 1 x 5 mL	1 × 5 mL	Get Quote
17549854	HiTrap MabSelect PrismA 5 x 5 mL	5 × 5 mL	Get Quote
17549851	HiTrap MabSelect PrismA 1 x 1 mL	1 × 1 mL	Get Quote
28967056	Protein A Mag Sepharose Xtra	2 x 1 ml	Get Quote
28967062	Protein A Mag Sepharose Xtra	5 x 1 ml	Get Quote
28903133	Protein A HP MultiTrap	4	Get Quote
28903132	Protein A HP SpinTrap	16 Columns	Get Quote
28985254	rProtein A GraviTrap		Get Quote
17040201	HiTrap Protein A HP, 5 x 1 ml	5 x 1 ml	Get Quote
17040203	HiTrap Protein A HP, 2 x 1 ml	2 x 1 ml	Get Quote
29048576	HiTrap Protein A HP, 1 x 1 ml	1 x 1 ml	Get Quote
28946489	HiTrap rProtein A FF 100 x 1 ml	100 x 1 ml
17507901	HiTrap rProtein A FF, 5 x 1 ml	5 x 1 ml	Get Quote
17507902	HiTrap rProtein A FF, 2 x 1 ml	2 x 1 ml	Get Quote
11003493	HiTrap MabSelect SuRe, 5 x 1 ml	5 x 1 ml	Get Quote
29049104	HiTrap MabSelect SuRe, 1 x 1 ml	1 x 1 ml	Get Quote
17549111	HiTrap MabSelect SuRe pcc, 5 x 1 ml	5 x 1 ml	Get Quote
17040301	HiTrap Protein A HP, 1 x 5 ml	1 x 5 ml	Get Quote
17040303	HiTrap Protein A HP, 5 x 5 ml	5 x 5 ml	Get Quote
17040503	HiTrap Protein G HP, 5 x 5 ml	5 x 5 ml	Get Quote
17508002	HiTrap rProtein A FF, 5 x 5 ml	5 x 5 ml	Get Quote
17508001	HiTrap rProtein A FF, 1 x 5 ml	1 x 5 ml	Get Quote
11003494	HiTrap MabSelect SuRe, 1 x 5 ml	1 x 5 ml	Get Quote
11003495	HiTrap MabSelect SuRe, 5 x 5 ml	5 x 5 ml	Get Quote
17549112	HiTrap MabSelect SuRe pcc, 1 x 5 ml	1 x 5 ml	Get Quote

Product	Product Name	Pack Size
17127901	rProtein A Sepharose Fast Flow	5 mL	Get Quote
17127902	rProtein A Sepharose Fast Flow	25 mL	Get Quote
17127903	rProtein A Sepharose Fast Flow	200 mL	Get Quote
17543801	MabSelect SuRe, 25 mL	25 mL	Get Quote
17543802	MabSelect SuRe, 200 mL	200 mL	Get Quote
17547401	MabSelect SuRe LX, 25 mL	25 mL	Get Quote
17547402	MabSelect SuRe LX, 200 mL	200 mL	Get Quote
17549101	MabSelect SuRe pcc, 25 mL	25 mL	Get Quote
17549102	MabSelect SuRe pcc, 200 mL	200 mL	Get Quote
17549801	MabSelect PrismA, 25 mL	25 mL	Get Quote
17549802	MabSelect PrismA, 200 mL	200 mL	Get Quote

Product	Product Name	Pack Size
17040201	HiTrap Protein A HP, 5 x 1 ml	5 x 1 ml	Get Quote
17040203	HiTrap Protein A HP, 2 x 1 ml	2 x 1 ml	Get Quote
29048576	HiTrap Protein A HP, 1 x 1 ml	1 x 1 ml	Get Quote
28946489	HiTrap rProtein A FF 100 x 1 ml	100 x 1 ml
17507901	HiTrap rProtein A FF, 5 x 1 ml	5 x 1 ml	Get Quote
17507902	HiTrap rProtein A FF, 2 x 1 ml	2 x 1 ml	Get Quote
11003493	HiTrap MabSelect SuRe, 5 x 1 ml	5 x 1 ml	Get Quote
29049104	HiTrap MabSelect SuRe, 1 x 1 ml	1 x 1 ml	Get Quote
17549111	HiTrap MabSelect SuRe pcc, 5 x 1 ml	5 x 1 ml	Get Quote
17040301	HiTrap Protein A HP, 1 x 5 ml	1 x 5 ml	Get Quote
17040303	HiTrap Protein A HP, 5 x 5 ml	5 x 5 ml	Get Quote
17040503	HiTrap Protein G HP, 5 x 5 ml	5 x 5 ml	Get Quote
17508002	HiTrap rProtein A FF, 5 x 5 ml	5 x 5 ml	Get Quote
17508001	HiTrap rProtein A FF, 1 x 5 ml	1 x 5 ml	Get Quote
11003494	HiTrap MabSelect SuRe, 1 x 5 ml	1 x 5 ml	Get Quote
11003495	HiTrap MabSelect SuRe, 5 x 5 ml	5 x 5 ml	Get Quote
17549112	HiTrap MabSelect SuRe pcc, 1 x 5 ml	1 x 5 ml	Get Quote
28926977	HiScreen MabSelect SuRe	1 × 4.7 mL	Get Quote
17547415	HiScreen MabSelect SuRe LX	1 × 4.7 mL	Get Quote
17549852	HiTrap MabSelect PrismA 5 x 1 mL	5 × 1 mL	Get Quote
17549853	HiTrap MabSelect PrismA 1 x 5 mL	1 × 5 mL	Get Quote
17549854	HiTrap MabSelect PrismA 5 x 5 mL	5 × 5 mL	Get Quote
17549815	HiScreen MabSelect PrismA	1 × 4.7 mL	Get Quote
17549851	HiTrap MabSelect PrismA 1 x 1 mL	1 × 1 mL	Get Quote

Product	Product Name	Pack Size
17061801	Protein G Sepharose 4 Fast Flow	5 mL	Get Quote
17061805	Protein G Sepharose 4 Fast Flow	200 mL	Get Quote
17061802	Protein G Sepharose 4 Fast Flow	25 mL	Get Quote
17088601	GammaBind Plus Sepharose, 5 mL	5 mL	Get Quote
17088602	GammaBind Plus Sepharose, 25 mL	25 mL	Get Quote
17088604	GammaBind Plus Sepharose, 500 mL	500 mL	Get Quote

Product	Product Name	Pack Size
28903135	Protein G HP MultiTrap	4	Get Quote
28408347	Ab SpinTrap	50 x 100 µl	Get Quote
28903134	Protein G HP SpinTrap	16 Columns	Get Quote
28985255	Protein G GraviTrap		Get Quote
28985256	rProtein A Protein G GraviTrap		Get Quote
17040403	HiTrap Protein G HP, 2 x 1 ml	2 x 1 ml	Get Quote
17040401	HiTrap Protein G HP, 5 x 1 ml	5 x 1 ml	Get Quote
29048581	HiTrap Protein G HP, 1 x 1 ml	1 x 1 ml	Get Quote
17040501	HiTrap Protein G HP, 1 x 5 ml	1 x 5 ml	Get Quote
17040503	HiTrap Protein G HP, 5 x 5 ml	5 x 5 ml	Get Quote

Product	Product Name	Pack Size
17061801	Protein G Sepharose 4 Fast Flow	5 mL	Get Quote
17061805	Protein G Sepharose 4 Fast Flow	200 mL	Get Quote
17061802	Protein G Sepharose 4 Fast Flow	25 mL	Get Quote
17088601	GammaBind Plus Sepharose, 5 mL	5 mL	Get Quote
17088602	GammaBind Plus Sepharose, 25 mL	25 mL	Get Quote
17088604	GammaBind Plus Sepharose, 500 mL	500 mL	Get Quote

Product	Product Name	Pack Size
17040403	HiTrap Protein G HP, 2 x 1 ml	2 x 1 ml	Get Quote
17040401	HiTrap Protein G HP, 5 x 1 ml	5 x 1 ml	Get Quote
29048581	HiTrap Protein G HP, 1 x 1 ml	1 x 1 ml	Get Quote
17040501	HiTrap Protein G HP, 1 x 5 ml	1 x 5 ml	Get Quote
17040503	HiTrap Protein G HP, 5 x 5 ml	5 x 5 ml	Get Quote
17511001	HiTrap IgM Purification HP	5 x 1 ml	Get Quote
17511101	HiTrap IgY Purification HP	5 ml	Get Quote

How do I choose between affinity media based on protein A or protein G?

Both protein A and protein G have high affinity and specificity for the Fc region of antibodies. However, they differ in their ability to bind antibodies of different species and subclasses (see table). We recommend protein A for purifying human antibodies. Protein G binds a broader range of IgG from eukaryotic species and binds more classes of IgG than protein A. Therefore, we recommend our protein G-based media to purify antibodies from other species, including rat. If you are not sure whether your antibody will bind to protein A or protein G, we suggest trying rProtein A/Protein G GraviTrap, which contains gravity flow columns packed with a combination of both media.

The table below shows the relative binding strengths of antibodies from various species to protein G and protein A.

Relative binding strengths of antibodies from various species to protein G and protein A as measured in a competitive ELISA test. The amount of IgG required to give a 50% inhibition of binding of rabbit IgG conjugated with alkaline phosphatase was determined.

Species	Subclass	Protein G binding	Protein A binding
* Purified using HiTrap IgM Purification HP columns ^† Purified using HiTrap IgY Purification HP columns ++++ = strong binding ++ = medium binding — = weak or no binding
Human	IgA	—	variable
	IgD	—	—
	IgE	—	—
	IgG₁	++++	++++
	IgG₂	++++	++++
	IgG₃	++++	—
	IgM*	—	variable
	IgG₄	++++	++++
Avian egg yolk	IgY^†	—	—
Cow		++++	++
Dog		+	++
Goat		++	—
Guinea pig	IgG₁	++	++++
Hamster		++	+
Horse		++++	++
Koala		+	—
Llama		+	—
Monkey (rhesus)		++++	++++
Mouse	IgG₁	++++	+
	IgG_2a	++++	++++
	IgG₂	+++	+++
	IgG₃	+++	++
	IgM*	—	variable
Pig		+++	+++
Rabbit		+++	++++
Rat	IgG₁	+	—
	IgG_2a	++++	—
	IgG_2b	++	—
	IgG₃	++	+
Sheep		++	+/—

What types of products does GE offer for affinity purification of antibodies and antibody fragments?

Our product offering includes a range of agarose-based media with protein A, protein G, or protein L as ligand. We provide a variety of protein A- and G-based products for purifying monoclonal and polyclonal antibodies from cell culture supernatants and serum. Capto L is our medium for purifying antibody fragments that contain a kappa light chain. These fragments include single-chain fragment variable (scFv), Fab, and domain antibodies (Dab). Most of our products are available as prepacked columns for use with chromatography systems, prepacked formats for manual purification, and loose media (resins). We also offer prepacked columns for IgM and IgY purification, which are based on thiophilic adsorption.

Please use our online selection tool to find a product that meets your needs.