Tagged Protein Purification Products - GE Healthcare Life Sciences

Tagged protein purification selection guide

To find the media or prepacked columns that suit your needs, choose the option below that best describes your application.

Tagged protein purification

Histidine-tag (his-tag)

GST-tag

Strep-tag™ //

MBP-tag

Product	Product Name	Pack Size
17513201	Glutathione Sepharose 4 Fast Flow, 25 mL	25 mL	Get Quote
17513202	Glutathione Sepharose 4 Fast Flow, 100 mL	100 mL	Get Quote
17513203	Glutathione Sepharose 4 Fast Flow, 500 mL	500 mL	Get Quote
17075601	Glutathione Sepharose 4B, 10 mL	10 mL	Get Quote
17075604	Glutathione Sepharose 4B, 300 mL	300 mL	Get Quote
17075605	Glutathione Sepharose 4B, 100 mL	100 mL	Get Quote
17527901	Glutathione Sepharose High Performance, 25 mL	25 mL	Get Quote
17527902	Glutathione Sepharose High Performance, 100 mL	100 mL	Get Quote
27457001	GST Bulk Kit	1 Kit	Get Quote

Product	Product Name	Pack Size
28405500	GST MultiTrap 4B	4 x 96-well filter plates	Get Quote
28952359	GST SpinTrap	50 x 50 µl	Get Quote
28952360	GST GraviTrap	10 x 2 ml	Get Quote
17513002	GSTrap FF	2 x 1 ml	Get Quote
17513005	GSTrap FF	100 x 1 ml
17513001	GSTrap FF	5 x 1 ml	Get Quote
28401746	GSTrap 4B, 100 x 1 ml	100 x 1 ml
28401745	GSTrap 4B, 5 x 1 ml	5 x 1 ml	Get Quote
29048609	GSTrap 4B, 1 x 1 ml	1 x 1 ml	Get Quote
17528101	GSTrap HP	5 x 1 ml	Get Quote
17528105	GSTrap HP	100 x 1 ml
17513101	GSTrap FF	1 x 5 ml	Get Quote
17513102	GSTrap FF	5 x 5 ml	Get Quote
17513105	GSTrap FF	100 x 5 ml
28401748	GSTrap 4B, 5 x 5 ml	5 x 5 ml	Get Quote
28401747	GSTrap 4B, 1 x 5 ml	1 x 5 ml	Get Quote
28401749	GSTrap 4B, 100 x 5 ml	100 x 5 ml
17528201	GSTrap HP	1 x 5 ml	Get Quote
17528202	GSTrap HP	5 x 5 ml	Get Quote
17528205	GSTrap HP	100 x 5 ml

Product	Product Name	Pack Size
17513201	Glutathione Sepharose 4 Fast Flow, 25 mL	25 mL	Get Quote
17513202	Glutathione Sepharose 4 Fast Flow, 100 mL	100 mL	Get Quote
17513203	Glutathione Sepharose 4 Fast Flow, 500 mL	500 mL	Get Quote
17075601	Glutathione Sepharose 4B, 10 mL	10 mL	Get Quote
17075604	Glutathione Sepharose 4B, 300 mL	300 mL	Get Quote
17075605	Glutathione Sepharose 4B, 100 mL	100 mL	Get Quote
17527901	Glutathione Sepharose High Performance, 25 mL	25 mL	Get Quote
17527902	Glutathione Sepharose High Performance, 100 mL	100 mL	Get Quote

Product	Product Name	Pack Size
17513002	GSTrap FF	2 x 1 ml	Get Quote
17513005	GSTrap FF	100 x 1 ml
17513001	GSTrap FF	5 x 1 ml	Get Quote
28401746	GSTrap 4B, 100 x 1 ml	100 x 1 ml
28401745	GSTrap 4B, 5 x 1 ml	5 x 1 ml	Get Quote
29048609	GSTrap 4B, 1 x 1 ml	1 x 1 ml	Get Quote
17528101	GSTrap HP	5 x 1 ml	Get Quote
17528105	GSTrap HP	100 x 1 ml
17513101	GSTrap FF	1 x 5 ml	Get Quote
17513102	GSTrap FF	5 x 5 ml	Get Quote
17513105	GSTrap FF	100 x 5 ml
28401748	GSTrap 4B, 5 x 5 ml	5 x 5 ml	Get Quote
28401747	GSTrap 4B, 1 x 5 ml	1 x 5 ml	Get Quote
28401749	GSTrap 4B, 100 x 5 ml	100 x 5 ml
17528201	GSTrap HP	1 x 5 ml	Get Quote
17528202	GSTrap HP	5 x 5 ml	Get Quote
17528205	GSTrap HP	100 x 5 ml
28936550	GSTPrep FF 16/10	20 ml	Get Quote

Product	Product Name	Pack Size
28935600	StrepTactin Sepharose High Performance, 50 mL	50 mL	Get Quote

Product	Product Name	Pack Size
28907546	StrepTrap HP, 5 x 1 ml	5 x 1 ml	Get Quote
29048653	StrepTrap HP, 1 x 1 ml	1 x 1 ml	Get Quote
28907548	StrepTrap HP, 5 x 5 ml	5 x 5 ml	Get Quote
28907547	StrepTrap HP, 1 x 5 ml	1 x 5 ml	Get Quote

Product	Product Name	Pack Size
28935600	StrepTactin Sepharose High Performance, 50 mL	50 mL	Get Quote

Product	Product Name	Pack Size
28907546	StrepTrap HP, 5 x 1 ml	5 x 1 ml	Get Quote
29048653	StrepTrap HP, 1 x 1 ml	1 x 1 ml	Get Quote
28907548	StrepTrap HP, 5 x 5 ml	5 x 5 ml	Get Quote
28907547	StrepTrap HP, 1 x 5 ml	1 x 5 ml	Get Quote

Product	Product Name	Pack Size
28935597	Dextrin Sepharose High Performance, 25 mL	25 mL	Get Quote
28935598	Dextrin Sepharose High Performance, 100 mL	100 mL	Get Quote

Product	Product Name	Pack Size
28918778	MBPTrap HP, 5 x 1 ml	5 x 1 ml	Get Quote
29048641	MBPTrap HP, 1 x 1 ml	1 x 1 ml	Get Quote
28918779	MBPTrap HP, 1 x 5 ml	1 x 5 ml	Get Quote
28918780	MBPTrap HP, 5 x 5 ml	5 x 5 ml	Get Quote

Product	Product Name	Pack Size
28935597	Dextrin Sepharose High Performance, 25 mL	25 mL	Get Quote
28935598	Dextrin Sepharose High Performance, 100 mL	100 mL	Get Quote

Product	Product Name	Pack Size
28918778	MBPTrap HP, 5 x 1 ml	5 x 1 ml	Get Quote
29048641	MBPTrap HP, 1 x 1 ml	1 x 1 ml	Get Quote
28918779	MBPTrap HP, 1 x 5 ml	1 x 5 ml	Get Quote
28918780	MBPTrap HP, 5 x 5 ml	5 x 5 ml	Get Quote

Product	Product Name	Pack Size
17531801	Ni Sepharose 6 Fast Flow, 25 mL	25 mL	Get Quote
17531806	Ni Sepharose 6 Fast Flow, 5 mL	5 mL	Get Quote
17531803	Ni Sepharose 6 Fast Flow, 500 mL	500 mL	Get Quote
17531802	Ni Sepharose 6 Fast Flow, 100 mL	100 mL	Get Quote
17526801	Ni Sepharose High Performance, 25 mL	25 mL	Get Quote
17526802	Ni Sepharose High Performance, 100 mL	100 mL	Get Quote
28957499	TALON Superflow, 10 mL	10 mL	Get Quote
28957502	TALON Superflow, 50 mL	50 mL	Get Quote
17371201	Ni Sepharose excel, 25 mL	25 mL	Get Quote
17371202	Ni Sepharose excel, 100 mL	100 mL	Get Quote
17371203	Ni Sepharose excel, 500 mL	500 mL	Get Quote

Product	Product Name	Pack Size
11000458	HisTrap FF Crude, 5 x 1 ml	5 x 1 ml	Get Quote
11000459	HisTrap FF Crude, 100 x 1 ml	100 x 1 ml
11003399	His GraviTrap	10 x 1 ml	Get Quote
17371205	HisTrap excel, 5 x 1 ml	5 x 1 ml	Get Quote
17371206	HisTrap excel, 5 x 5 ml	5 x 5 ml	Get Quote
17371220	His Mag Sepharose excel	2 x 1 ml	Get Quote
17371221	His Mag Sepharose excel	5 x 1 ml	Get Quote
17371222	His Mag Sepharose excel	10 x 1 ml	Get Quote
17524701	HisTrap HP, 5 x 1 ml	5 x 1 ml	Get Quote
17524705	HisTrap HP, 100 x 1 ml	100 x 1 ml
17524801	HisTrap HP, 1 x 5 ml	1 x 5 ml	Get Quote
17524802	HisTrap HP, 5 x 5 ml	5 x 5 ml	Get Quote
17524805	HisTrap HP, 100 x 5 ml	100 x 5 ml
17525501	HisTrap FF	5 x 5 ml	Get Quote
17525502	HisTrap FF	100 x 5 ml
17528601	HisTrap FF Crude, 5 x 5 ml	5 x 5 ml	Get Quote
17528602	HisTrap FF Crude, 100 x 5 ml	100 x 5 ml
17531901	HisTrap FF	5 x 1 ml	Get Quote
17531902	HisTrap FF	100 x 1 ml
28400989	His MultiTrap HP	4 x 96-well filter plates	Get Quote
28400990	His MultiTrap FF	4 x 96-well filter plates	Get Quote
28401351	His GraviTrap Kit	10 Columns	Get Quote
28401353	His SpinTrap	50 x 100 µl	Get Quote
28932171	His SpinTrap Kit	1 Piece	Get Quote
28953766	HiTrap TALON crude, 5 x 1 ml	5 x 1 ml	Get Quote
28953767	HiTrap TALON crude, 5 x 5 ml	5 x 5 ml	Get Quote
28953805	HiTrap TALON crude, 100 x 1 ml	100 x 1 ml
28953809	HiTrap TALON crude, 100 x 5 ml	100 x 5 ml
28967388	His Mag Sepharose Ni	2 x 1 ml	Get Quote
28967390	His Mag Sepharose Ni	5 x 1 ml	Get Quote
28979917	His Mag Sepharose Ni	10 x 1 ml	Get Quote
29000593	His SpinTrap TALON	50 x 100 μl	Get Quote
29000594	His GraviTrap TALON	10	Get Quote
29000596	His MultiTrap TALON	4 x 96-well filter plates	Get Quote
29048565	HiTrap TALON crude, 1 x 1 ml	1 x 1 ml	Get Quote
29048586	HisTrap excel, 1 x 1 ml	1 x 1 ml	Get Quote
29048631	HisTrap FF Crude, 1 x 1 ml	1 x 1 ml	Get Quote
29051021	HisTrap HP, 1 x 1 ml	1 x 1 ml	Get Quote
29104065	His Mag Sepharose Ni	100 ml	Get Quote

Product	Product Name	Pack Size
17531801	Ni Sepharose 6 Fast Flow, 25 mL	25 mL	Get Quote
17531806	Ni Sepharose 6 Fast Flow, 5 mL	5 mL	Get Quote
17531803	Ni Sepharose 6 Fast Flow, 500 mL	500 mL	Get Quote
17531802	Ni Sepharose 6 Fast Flow, 100 mL	100 mL	Get Quote
17526801	Ni Sepharose High Performance, 25 mL	25 mL	Get Quote
17526802	Ni Sepharose High Performance, 100 mL	100 mL	Get Quote
28957499	TALON Superflow, 10 mL	10 mL	Get Quote
28957502	TALON Superflow, 50 mL	50 mL	Get Quote
17371201	Ni Sepharose excel, 25 mL	25 mL	Get Quote
17371202	Ni Sepharose excel, 100 mL	100 mL	Get Quote
17371203	Ni Sepharose excel, 500 mL	500 mL	Get Quote

Product	Product Name	Pack Size
29048631	HisTrap FF Crude, 1 x 1 ml	1 x 1 ml	Get Quote
11000458	HisTrap FF Crude, 5 x 1 ml	5 x 1 ml	Get Quote
11000459	HisTrap FF Crude, 100 x 1 ml	100 x 1 ml
29048565	HiTrap TALON crude, 1 x 1 ml	1 x 1 ml	Get Quote
28953766	HiTrap TALON crude, 5 x 1 ml	5 x 1 ml	Get Quote
17528601	HisTrap FF Crude, 5 x 5 ml	5 x 5 ml	Get Quote
17528602	HisTrap FF Crude, 100 x 5 ml	100 x 5 ml
28953809	HiTrap TALON crude, 100 x 5 ml	100 x 5 ml
17531901	HisTrap FF	5 x 1 ml	Get Quote
17531902	HisTrap FF	100 x 1 ml
29051021	HisTrap HP, 1 x 1 ml	1 x 1 ml	Get Quote
17524701	HisTrap HP, 5 x 1 ml	5 x 1 ml	Get Quote
17524705	HisTrap HP, 100 x 1 ml	100 x 1 ml
17371205	HisTrap excel, 5 x 1 ml	5 x 1 ml	Get Quote
29048586	HisTrap excel, 1 x 1 ml	1 x 1 ml	Get Quote
28953805	HiTrap TALON crude, 100 x 1 ml	100 x 1 ml
17525501	HisTrap FF	5 x 5 ml	Get Quote
17525502	HisTrap FF	100 x 5 ml
17524801	HisTrap HP, 1 x 5 ml	1 x 5 ml	Get Quote
17524802	HisTrap HP, 5 x 5 ml	5 x 5 ml	Get Quote
17524805	HisTrap HP, 100 x 5 ml	100 x 5 ml
17371206	HisTrap excel, 5 x 5 ml	5 x 5 ml	Get Quote
28953767	HiTrap TALON crude, 5 x 5 ml	5 x 5 ml	Get Quote
28936551	HisPrep FF 16/10	20 ml	Get Quote
28978244	HiScreen Ni FF	4.7 ml	Get Quote

Product	Product Name	Pack Size
17092107	IMAC Sepharose 6 Fast Flow, 25 mL	25 mL	Get Quote
17092108	IMAC Sepharose 6 Fast Flow, 100 mL	100 mL	Get Quote
17092007	IMAC Sepharose High Performance, 100 mL	100 mL	Get Quote

Product	Product Name	Pack Size
17092102	HiTrap IMAC FF	5 x 1 ml	Get Quote
17092003	HiTrap IMAC HP	5 x 1 ml	Get Quote
17092104	HiTrap IMAC FF	5 x 5 ml	Get Quote
17092005	HiTrap IMAC HP	5 x 5 ml	Get Quote

Product	Product Name	Pack Size
17092107	IMAC Sepharose 6 Fast Flow, 25 mL	25 mL	Get Quote
17092108	IMAC Sepharose 6 Fast Flow, 100 mL	100 mL	Get Quote
17092007	IMAC Sepharose High Performance, 100 mL	100 mL	Get Quote

Product	Product Name	Pack Size
17092102	HiTrap IMAC FF	5 x 1 ml	Get Quote
17092003	HiTrap IMAC HP	5 x 1 ml	Get Quote
17092104	HiTrap IMAC FF	5 x 5 ml	Get Quote
17092005	HiTrap IMAC HP	5 x 5 ml	Get Quote
28936552	HiPrep IMAC FF 16/10	20 ml	Get Quote
28978244	HiScreen Ni FF	4.7 ml	Get Quote

What types of affinity products does GE offer for histidine-tagged protein purification?

We offer a variety of products containing IMAC media precharged with nickel or cobalt ions. These products include prepacked columns for use with chromatography systems, prepacked formats for manual purification, and loose media (resins). Options containing uncharged IMAC media are also available. We recommend products containing the following media types based on your purification priorities:

High purity: TALON Superflow, which is precharged with cobalt ions
Avoiding nickel leakage: Ni Sepharose excel, which is precharged with nickel ions
High binding capacity: Ni Sepharose High Performance, which is precharged with nickel ions
Ability to scale up purification: Ni Sepharose 6 Fast Flow, which is precharged with nickel ions
Flexibility in metal ions used: Uncharged IMAC media

How do I choose between precharged and uncharged media for histidine-tagged protein purification?

The choice of metal ion affects binding specificity and strength. The requirements for this purification step will help with this choice. Nickel (Ni²⁺) is the most popular metal ion for histidine-tagged (his-tagged) protein purification. But nickel tends to bind nonspecifically to host proteins containing histidine clusters. Cobalt (Co²⁺) interacts more specifically with his-tags, often giving a more pure protein. GE offers many products containing media precharged with nickel or cobalt ions. Uncharged IMAC media are flexible options for preparing media charged with metal ions other than nickel or cobalt, such as zinc or copper.

Should I be concerned about metal ion stripping during histidine-tagged protein purification?

Mammalian (e.g., CHO cells) and insect cell culture media contain substances that could reduce the yield of purified histidine-tagged (his-tagged) proteins. These components lower the binding to the IMAC medium by stripping the chelated metal ion. To improve protein yield, the sample can be pretreated to remove the interfering substances. Another option is to use a medium (e.g., Ni Sepharose excel) that binds to his-tagged proteins in the presence of metal stripping agents.

Does my sample always have to be clarified before histidine-tagged protein purification using a column?

Before applying a crude sample to an IMAC column, the sample is typically clarified using centrifugation and filtration. An alternative is to apply an unclarified, crude sample to an appropriate column directly after thorough cell disruption. This option can save time and help to minimize protein degradation. GE provides a wide range of products that have been designed to purify histidine-tagged proteins from unclarified samples.