Tagged Protein Purification Products
Selection guides to help you select the right chromatography media and prepacked columns
Tagged protein purification selection guide
To find the media or prepacked columns that suit your needs, choose the option below that best describes your application.
Tagged protein purification
Histidine-tag (his-tag)
GST-tag
Strep-tag™ //
MBP-tag
Will you use media precharged with a metal ion or uncharged media?
Precharged
Uncharged
Will you use a chromatography system or manual purification?
Manual purification
Chromatography system
Will you use a chromatography system or manual purification?
Manual purification
Chromatography system
Will you use a chromatography system or manual purification?
Manual purification
Chromatography system
Will you use a chromatography system or manual purification?
Manual purification
Chromatography system
Will you use a chromatography system or manual purification?
Manual purification
Chromatography system
What format do you wish to use?
Loose resin
Prepacked columns and other formats
What format do you wish to use?
Loose resin
Prepacked columns and other formats
What format do you wish to use?
Loose resin
Prepacked columns and other formats
What format do you wish to use?
Loose resin
Prepacked columns and other formats
What format do you wish to use?
Loose resin
Prepacked columns and other formats
What format do you wish to use?
Loose media (resin)
Prepacked columns and other formats
What format do you wish to use?
Loose resin
Prepacked columns and other formats
What format do you wish to use?
Loose resin
Prepacked columns and other formats
What format do you wish to use?
Loose resin
Prepacked columns and other formats
What format do you wish to use?
Loose resin
Prepacked columns and other formats
| Product | Product Name | Pack Size | |
|---|---|---|---|
| 17513201 | Glutathione Sepharose 4 Fast Flow, 25 mL | 25 mL | Add to Cart |
| 17513202 | Glutathione Sepharose 4 Fast Flow, 100 mL | 100 mL | Add to Cart |
| 17513203 | Glutathione Sepharose 4 Fast Flow, 500 mL | 500 mL | Add to Cart |
| 17075601 | Glutathione Sepharose 4B, 10 mL | 10 mL | Add to Cart |
| 17075604 | Glutathione Sepharose 4B, 300 mL | 300 mL | Add to Cart |
| 17075605 | Glutathione Sepharose 4B, 100 mL | 100 mL | Add to Cart |
| 17527901 | Glutathione Sepharose High Performance, 25 mL | 25 mL | Add to Cart |
| 17527902 | Glutathione Sepharose High Performance, 100 mL | 100 mL | Add to Cart |
| 27457001 | GST Bulk Kit | 1 Kit | Add to Cart |
| Product | Product Name | Pack Size | |
|---|---|---|---|
| 28405500 | GST MultiTrap 4B | 4 x 96-well filter plates | Add to Cart |
| 28952359 | GST SpinTrap | 50 x 50 µl | Add to Cart |
| 28952360 | GST GraviTrap | 10 x 2 ml | Add to Cart |
| 17513002 | GSTrap FF | 2 x 1 ml | Add to Cart |
| 17513005 | GSTrap FF | 100 x 1 ml | |
| 17513001 | GSTrap FF | 5 x 1 ml | Add to Cart |
| 28401746 | GSTrap 4B, 100 x 1 ml | 100 x 1 ml | |
| 28401745 | GSTrap 4B, 5 x 1 ml | 5 x 1 ml | Add to Cart |
| 29048609 | GSTrap 4B, 1 x 1 ml | 1 x 1 ml | Add to Cart |
| 17528101 | GSTrap HP | 5 x 1 ml | Add to Cart |
| 17528105 | GSTrap HP | 100 x 1 ml | |
| 17513101 | GSTrap FF | 1 x 5 ml | Add to Cart |
| 17513102 | GSTrap FF | 5 x 5 ml | Add to Cart |
| 17513105 | GSTrap FF | 100 x 5 ml | |
| 28401748 | GSTrap 4B, 5 x 5 ml | 5 x 5 ml | Add to Cart |
| 28401747 | GSTrap 4B, 1 x 5 ml | 1 x 5 ml | Add to Cart |
| 28401749 | GSTrap 4B, 100 x 5 ml | 100 x 5 ml | |
| 17528201 | GSTrap HP | 1 x 5 ml | Add to Cart |
| 17528202 | GSTrap HP | 5 x 5 ml | Add to Cart |
| 17528205 | GSTrap HP | 100 x 5 ml |
| Product | Product Name | Pack Size | |
|---|---|---|---|
| 17513201 | Glutathione Sepharose 4 Fast Flow, 25 mL | 25 mL | Add to Cart |
| 17513202 | Glutathione Sepharose 4 Fast Flow, 100 mL | 100 mL | Add to Cart |
| 17513203 | Glutathione Sepharose 4 Fast Flow, 500 mL | 500 mL | Add to Cart |
| 17075601 | Glutathione Sepharose 4B, 10 mL | 10 mL | Add to Cart |
| 17075604 | Glutathione Sepharose 4B, 300 mL | 300 mL | Add to Cart |
| 17075605 | Glutathione Sepharose 4B, 100 mL | 100 mL | Add to Cart |
| 17527901 | Glutathione Sepharose High Performance, 25 mL | 25 mL | Add to Cart |
| 17527902 | Glutathione Sepharose High Performance, 100 mL | 100 mL | Add to Cart |
| Product | Product Name | Pack Size | |
|---|---|---|---|
| 17513002 | GSTrap FF | 2 x 1 ml | Add to Cart |
| 17513005 | GSTrap FF | 100 x 1 ml | |
| 17513001 | GSTrap FF | 5 x 1 ml | Add to Cart |
| 28401746 | GSTrap 4B, 100 x 1 ml | 100 x 1 ml | |
| 28401745 | GSTrap 4B, 5 x 1 ml | 5 x 1 ml | Add to Cart |
| 29048609 | GSTrap 4B, 1 x 1 ml | 1 x 1 ml | Add to Cart |
| 17528101 | GSTrap HP | 5 x 1 ml | Add to Cart |
| 17528105 | GSTrap HP | 100 x 1 ml | |
| 17513101 | GSTrap FF | 1 x 5 ml | Add to Cart |
| 17513102 | GSTrap FF | 5 x 5 ml | Add to Cart |
| 17513105 | GSTrap FF | 100 x 5 ml | |
| 28401748 | GSTrap 4B, 5 x 5 ml | 5 x 5 ml | Add to Cart |
| 28401747 | GSTrap 4B, 1 x 5 ml | 1 x 5 ml | Add to Cart |
| 28401749 | GSTrap 4B, 100 x 5 ml | 100 x 5 ml | |
| 17528201 | GSTrap HP | 1 x 5 ml | Add to Cart |
| 17528202 | GSTrap HP | 5 x 5 ml | Add to Cart |
| 17528205 | GSTrap HP | 100 x 5 ml | |
| 28936550 | GSTPrep FF 16/10 | 20 ml | Add to Cart |
| Product | Product Name | Pack Size | |
|---|---|---|---|
| 28935600 | StrepTactin Sepharose High Performance, 50 mL | 50 mL | Add to Cart |
| Product | Product Name | Pack Size | |
|---|---|---|---|
| 28907546 | StrepTrap HP, 5 x 1 ml | 5 x 1 ml | Add to Cart |
| 29048653 | StrepTrap HP, 1 x 1 ml | 1 x 1 ml | Add to Cart |
| 28907548 | StrepTrap HP, 5 x 5 ml | 5 x 5 ml | Add to Cart |
| 28907547 | StrepTrap HP, 1 x 5 ml | 1 x 5 ml | Add to Cart |
| Product | Product Name | Pack Size | |
|---|---|---|---|
| 28935600 | StrepTactin Sepharose High Performance, 50 mL | 50 mL | Add to Cart |
| Product | Product Name | Pack Size | |
|---|---|---|---|
| 28907546 | StrepTrap HP, 5 x 1 ml | 5 x 1 ml | Add to Cart |
| 29048653 | StrepTrap HP, 1 x 1 ml | 1 x 1 ml | Add to Cart |
| 28907548 | StrepTrap HP, 5 x 5 ml | 5 x 5 ml | Add to Cart |
| 28907547 | StrepTrap HP, 1 x 5 ml | 1 x 5 ml | Add to Cart |
| Product | Product Name | Pack Size | |
|---|---|---|---|
| 28935597 | Dextrin Sepharose High Performance, 25 mL | 25 mL | Add to Cart |
| 28935598 | Dextrin Sepharose High Performance, 100 mL | 100 mL | Add to Cart |
| Product | Product Name | Pack Size | |
|---|---|---|---|
| 28918778 | MBPTrap HP, 5 x 1 ml | 5 x 1 ml | Add to Cart |
| 29048641 | MBPTrap HP, 1 x 1 ml | 1 x 1 ml | Add to Cart |
| 28918779 | MBPTrap HP, 1 x 5 ml | 1 x 5 ml | Add to Cart |
| 28918780 | MBPTrap HP, 5 x 5 ml | 5 x 5 ml | Add to Cart |
| Product | Product Name | Pack Size | |
|---|---|---|---|
| 28935597 | Dextrin Sepharose High Performance, 25 mL | 25 mL | Add to Cart |
| 28935598 | Dextrin Sepharose High Performance, 100 mL | 100 mL | Add to Cart |
| Product | Product Name | Pack Size | |
|---|---|---|---|
| 28918778 | MBPTrap HP, 5 x 1 ml | 5 x 1 ml | Add to Cart |
| 29048641 | MBPTrap HP, 1 x 1 ml | 1 x 1 ml | Add to Cart |
| 28918779 | MBPTrap HP, 1 x 5 ml | 1 x 5 ml | Add to Cart |
| 28918780 | MBPTrap HP, 5 x 5 ml | 5 x 5 ml | Add to Cart |
| Product | Product Name | Pack Size | |
|---|---|---|---|
| 17531801 | Ni Sepharose 6 Fast Flow, 25 mL | 25 mL | Add to Cart |
| 17531806 | Ni Sepharose 6 Fast Flow, 5 mL | 5 mL | Add to Cart |
| 17531803 | Ni Sepharose 6 Fast Flow, 500 mL | 500 mL | Add to Cart |
| 17531802 | Ni Sepharose 6 Fast Flow, 100 mL | 100 mL | Add to Cart |
| 17526801 | Ni Sepharose High Performance, 25 mL | 25 mL | Add to Cart |
| 17526802 | Ni Sepharose High Performance, 100 mL | 100 mL | Add to Cart |
| 28957499 | TALON Superflow, 10 mL | 10 mL | Add to Cart |
| 28957502 | TALON Superflow, 50 mL | 50 mL | Add to Cart |
| 17371201 | Ni Sepharose excel, 25 mL | 25 mL | Add to Cart |
| 17371202 | Ni Sepharose excel, 100 mL | 100 mL | Add to Cart |
| 17371203 | Ni Sepharose excel, 500 mL | 500 mL | Add to Cart |
| Product | Product Name | Pack Size | |
|---|---|---|---|
| 11000458 | HisTrap FF Crude, 5 x 1 ml | 5 x 1 ml | Add to Cart |
| 11000459 | HisTrap FF Crude, 100 x 1 ml | 100 x 1 ml | |
| 11003399 | His GraviTrap | 10 x 1 ml | Add to Cart |
| 17371205 | HisTrap excel, 5 x 1 ml | 5 x 1 ml | Add to Cart |
| 17371206 | HisTrap excel, 5 x 5 ml | 5 x 5 ml | Add to Cart |
| 17371220 | His Mag Sepharose excel | 2 x 1 ml | Add to Cart |
| 17371221 | His Mag Sepharose excel | 5 x 1 ml | Add to Cart |
| 17371222 | His Mag Sepharose excel | 10 x 1 ml | Add to Cart |
| 17524701 | HisTrap HP, 5 x 1 ml | 5 x 1 ml | Add to Cart |
| 17524705 | HisTrap HP, 100 x 1 ml | 100 x 1 ml | |
| 17524801 | HisTrap HP, 1 x 5 ml | 1 x 5 ml | Add to Cart |
| 17524802 | HisTrap HP, 5 x 5 ml | 5 x 5 ml | Add to Cart |
| 17524805 | HisTrap HP, 100 x 5 ml | 100 x 5 ml | |
| 17525501 | HisTrap FF | 5 x 5 ml | Add to Cart |
| 17525502 | HisTrap FF | 100 x 5 ml | |
| 17528601 | HisTrap FF Crude, 5 x 5 ml | 5 x 5 ml | Add to Cart |
| 17528602 | HisTrap FF Crude, 100 x 5 ml | 100 x 5 ml | |
| 17531901 | HisTrap FF | 5 x 1 ml | Add to Cart |
| 17531902 | HisTrap FF | 100 x 1 ml | |
| 28400989 | His MultiTrap HP | 4 x 96-well filter plates | Add to Cart |
| 28400990 | His MultiTrap FF | 4 x 96-well filter plates | Add to Cart |
| 28401351 | His GraviTrap Kit | 10 Columns | Add to Cart |
| 28401353 | His SpinTrap | 50 x 100 µl | Add to Cart |
| 28932171 | His SpinTrap Kit | 1 Piece | Add to Cart |
| 28953766 | HiTrap TALON crude, 5 x 1 ml | 5 x 1 ml | Add to Cart |
| 28953767 | HiTrap TALON crude, 5 x 5 ml | 5 x 5 ml | Add to Cart |
| 28953805 | HiTrap TALON crude, 100 x 1 ml | 100 x 1 ml | |
| 28953809 | HiTrap TALON crude, 100 x 5 ml | 100 x 5 ml | |
| 28967388 | His Mag Sepharose Ni | 2 x 1 ml | Add to Cart |
| 28967390 | His Mag Sepharose Ni | 5 x 1 ml | Add to Cart |
| 28979917 | His Mag Sepharose Ni | 10 x 1 ml | Get Quote |
| 29000593 | His SpinTrap TALON | 50 x 100 μl | Add to Cart |
| 29000594 | His GraviTrap TALON | 10 | Add to Cart |
| 29000596 | His MultiTrap TALON | 4 x 96-well filter plates | Add to Cart |
| 29048565 | HiTrap TALON crude, 1 x 1 ml | 1 x 1 ml | Add to Cart |
| 29048586 | HisTrap excel, 1 x 1 ml | 1 x 1 ml | Add to Cart |
| 29048631 | HisTrap FF Crude, 1 x 1 ml | 1 x 1 ml | Add to Cart |
| 29051021 | HisTrap HP, 1 x 1 ml | 1 x 1 ml | Add to Cart |
| 29104065 | His Mag Sepharose Ni | 100 ml | Get Quote |
| Product | Product Name | Pack Size | |
|---|---|---|---|
| 17531801 | Ni Sepharose 6 Fast Flow, 25 mL | 25 mL | Add to Cart |
| 17531806 | Ni Sepharose 6 Fast Flow, 5 mL | 5 mL | Add to Cart |
| 17531803 | Ni Sepharose 6 Fast Flow, 500 mL | 500 mL | Add to Cart |
| 17531802 | Ni Sepharose 6 Fast Flow, 100 mL | 100 mL | Add to Cart |
| 17526801 | Ni Sepharose High Performance, 25 mL | 25 mL | Add to Cart |
| 17526802 | Ni Sepharose High Performance, 100 mL | 100 mL | Add to Cart |
| 28957499 | TALON Superflow, 10 mL | 10 mL | Add to Cart |
| 28957502 | TALON Superflow, 50 mL | 50 mL | Add to Cart |
| 17371201 | Ni Sepharose excel, 25 mL | 25 mL | Add to Cart |
| 17371202 | Ni Sepharose excel, 100 mL | 100 mL | Add to Cart |
| 17371203 | Ni Sepharose excel, 500 mL | 500 mL | Add to Cart |
| Product | Product Name | Pack Size | |
|---|---|---|---|
| 29048631 | HisTrap FF Crude, 1 x 1 ml | 1 x 1 ml | Add to Cart |
| 11000458 | HisTrap FF Crude, 5 x 1 ml | 5 x 1 ml | Add to Cart |
| 11000459 | HisTrap FF Crude, 100 x 1 ml | 100 x 1 ml | |
| 29048565 | HiTrap TALON crude, 1 x 1 ml | 1 x 1 ml | Add to Cart |
| 28953766 | HiTrap TALON crude, 5 x 1 ml | 5 x 1 ml | Add to Cart |
| 17528601 | HisTrap FF Crude, 5 x 5 ml | 5 x 5 ml | Add to Cart |
| 17528602 | HisTrap FF Crude, 100 x 5 ml | 100 x 5 ml | |
| 28953809 | HiTrap TALON crude, 100 x 5 ml | 100 x 5 ml | |
| 17531901 | HisTrap FF | 5 x 1 ml | Add to Cart |
| 17531902 | HisTrap FF | 100 x 1 ml | |
| 29051021 | HisTrap HP, 1 x 1 ml | 1 x 1 ml | Add to Cart |
| 17524701 | HisTrap HP, 5 x 1 ml | 5 x 1 ml | Add to Cart |
| 17524705 | HisTrap HP, 100 x 1 ml | 100 x 1 ml | |
| 17371205 | HisTrap excel, 5 x 1 ml | 5 x 1 ml | Add to Cart |
| 29048586 | HisTrap excel, 1 x 1 ml | 1 x 1 ml | Add to Cart |
| 28953805 | HiTrap TALON crude, 100 x 1 ml | 100 x 1 ml | |
| 17525501 | HisTrap FF | 5 x 5 ml | Add to Cart |
| 17525502 | HisTrap FF | 100 x 5 ml | |
| 17524801 | HisTrap HP, 1 x 5 ml | 1 x 5 ml | Add to Cart |
| 17524802 | HisTrap HP, 5 x 5 ml | 5 x 5 ml | Add to Cart |
| 17524805 | HisTrap HP, 100 x 5 ml | 100 x 5 ml | |
| 17371206 | HisTrap excel, 5 x 5 ml | 5 x 5 ml | Add to Cart |
| 28953767 | HiTrap TALON crude, 5 x 5 ml | 5 x 5 ml | Add to Cart |
| 28936551 | HisPrep FF 16/10 | 20 ml | Add to Cart |
| 28978244 | HiScreen Ni FF | 4.7 ml | Add to Cart |
| Product | Product Name | Pack Size | |
|---|---|---|---|
| 17092107 | IMAC Sepharose 6 Fast Flow, 25 mL | 25 mL | Add to Cart |
| 17092108 | IMAC Sepharose 6 Fast Flow, 100 mL | 100 mL | Add to Cart |
| 17092007 | IMAC Sepharose High Performance, 100 mL | 100 mL | Add to Cart |
| Product | Product Name | Pack Size | |
|---|---|---|---|
| 17092102 | HiTrap IMAC FF | 5 x 1 ml | Add to Cart |
| 17092003 | HiTrap IMAC HP | 5 x 1 ml | Add to Cart |
| 17092104 | HiTrap IMAC FF | 5 x 5 ml | Add to Cart |
| 17092005 | HiTrap IMAC HP | 5 x 5 ml | Add to Cart |
| Product | Product Name | Pack Size | |
|---|---|---|---|
| 17092107 | IMAC Sepharose 6 Fast Flow, 25 mL | 25 mL | Add to Cart |
| 17092108 | IMAC Sepharose 6 Fast Flow, 100 mL | 100 mL | Add to Cart |
| 17092007 | IMAC Sepharose High Performance, 100 mL | 100 mL | Add to Cart |
| Product | Product Name | Pack Size | |
|---|---|---|---|
| 17092102 | HiTrap IMAC FF | 5 x 1 ml | Add to Cart |
| 17092003 | HiTrap IMAC HP | 5 x 1 ml | Add to Cart |
| 17092104 | HiTrap IMAC FF | 5 x 5 ml | Add to Cart |
| 17092005 | HiTrap IMAC HP | 5 x 5 ml | Add to Cart |
| 28936552 | HiPrep IMAC FF 16/10 | 20 ml | Add to Cart |
| 28978244 | HiScreen Ni FF | 4.7 ml | Add to Cart |
Request for quote
Oops! Something went wrong! Help us improve your experience by sending an error report.
Thank you. We will be in touch with you shortly.
We offer a variety of products containing IMAC media precharged with nickel or cobalt ions. These products include prepacked columns for use with chromatography systems, prepacked formats for manual purification, and loose media (resins). Options containing uncharged IMAC media are also available. We recommend products containing the following media types based on your purification priorities:
- High purity: TALON Superflow, which is precharged with cobalt ions
- Avoiding nickel leakage: Ni Sepharose excel, which is precharged with nickel ions
- High binding capacity: Ni Sepharose High Performance, which is precharged with nickel ions
- Ability to scale up purification: Ni Sepharose 6 Fast Flow, which is precharged with nickel ions
- Flexibility in metal ions used: Uncharged IMAC media
The choice of metal ion affects binding specificity and strength. The requirements for this purification step will help with this choice. Nickel (Ni2+) is the most popular metal ion for histidine-tagged (his-tagged) protein purification. But nickel tends to bind nonspecifically to host proteins containing histidine clusters. Cobalt (Co2+) interacts more specifically with his-tags, often giving a more pure protein. GE offers many products containing media precharged with nickel or cobalt ions. Uncharged IMAC media are flexible options for preparing media charged with metal ions other than nickel or cobalt, such as zinc or copper.
Mammalian (e.g., CHO cells) and insect cell culture media contain substances that could reduce the yield of purified histidine-tagged (his-tagged) proteins. These components lower the binding to the IMAC medium by stripping the chelated metal ion. To improve protein yield, the sample can be pretreated to remove the interfering substances. Another option is to use a medium (e.g., Ni Sepharose excel) that binds to his-tagged proteins in the presence of metal stripping agents.
Before applying a crude sample to an IMAC column, the sample is typically clarified using centrifugation and filtration. An alternative is to apply an unclarified, crude sample to an appropriate column directly after thorough cell disruption. This option can save time and help to minimize protein degradation. GE provides a wide range of products that have been designed to purify histidine-tagged proteins from unclarified samples.